AffiNGS® VAHTS Universal V8 RNA-seq Library Prep Kit for MGI

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CAT# AFG-VZ-NRM605

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    AffiNGS® VAHTS Universal V8 RNA-seq Library Prep Kit for MGI

    AffiNGS® VAHTS Universal V8 RNA-seq Library Prep Kit for MGI is specially designed for the preparation of transcriptome libraries for next generation sequencing (NGS) platforms of MGI®. The kit is universal and suitable for RNA library construction of RNA that have been obtained by Poly(A)-based mRNA enrichment or rRNA depletion. The kit contains two types of cDNA 2nd Strand synthesis buffer, which can be chosen for library construction for non-stranded or stranded RNA-Seq transcriptome analysis.
    This kit combines 2nd Strand cDNA synthesis, end-repair and dA-Tailing into one step, with
    no need of purification, which greatly simplifies the process of library construction and shortens the operation time. The optimized reaction system improves the library construction efficiency, is compatible with lower-input RNA, and has uniform coverage for different amounts of input-RNA. Libraries of specific sizes, which can be customized, can be obtained after size selection with magnetic beads. All the enzymes and buffer provided in the kit have undergone rigorous quality control and functional testing to ensure the optimal stability and repeatability.

     Components:

    ComponentsAFG-VZ-NRM605-01
    (24 rxns)
    AFG-VZ-NRM605-02
    (96 rxns)
    2 X Frag/Prime Buffer240 µl960 µl
    1st Strand Buffer 3168 µl672 µl
    1st Strand Enzyme Mix 348 µl192 µl
    2nd Strand Buffer 2 (with dNTP)600 µl4 X 600 µl
    2nd Strand Buffer 2 (with dNTP)600 µl4 X 600 µl
    2nd strand Enzyme super Mix 2360 µl2 X 720 µl
    Rapid Ligation Buffer 4600 µl4 X 600 µl
    Rapid DNA Ligase 4120 µl480 µl
    PCR Primer Mix 4 120 µl480 µl
    2 X HF Amplification Mix 600 µl4 X 600 µl

     

    Storage:
    Store at -30 ~ -15°C and transport at ≤ 0°C.