NGS Kits

Next-Generation Sequencing (NGS) Kits are comprehensive reagent systems designed to prepare DNA or RNA samples for sequencing on high-throughput platforms. These kits streamline critical steps such as nucleic acid extraction, library preparation, target enrichment, and indexing, ensuring high-quality and reproducible data for applications ranging from genomics to transcriptomics.

Content

• Principle of NGS Workflow
  The NGS workflow involves several key steps:
  • Nucleic Acid Extraction: Isolating high-quality DNA or RNA from various sample types.
  • Library Preparation: Fragmenting nucleic acids and attaching sequencing adapters.
  • Amplification: Enriching target sequences for improved coverage and sensitivity.
  • Sequencing: High-throughput analysis of prepared libraries.
  • Data Analysis: Bioinformatics processing of raw data into meaningful insights.
• Types of NGS Kits
  • Library Preparation Kits:
    Used to convert DNA/RNA into sequencing-ready libraries. Examples include kits for whole genome sequencing (WGS), whole exome sequencing (WES), or RNA-seq.
  • Target Enrichment Kits:
    Focus on specific genomic regions of interest using hybridization or amplicon-based methods.
  • Indexing Kits:
    Provide barcodes for multiplexing multiple samples in a single sequencing run.
  • Quality Control Kits:
    Ensure nucleic acid integrity and library quantification prior to sequencing.

Applications of NGS Kits

• Genomics
  • Whole genome sequencing (WGS) for comprehensive analysis of genetic variation.
  • Whole exome sequencing (WES) to study protein-coding regions associated with diseases.
• Transcriptomics
  • RNA-seq for profiling gene expression, alternative splicing, and non-coding RNAs.
  • Single-cell RNA sequencing (scRNA-seq) for cellular heterogeneity studies.
• Epigenomics
  • Methyl-seq for DNA methylation profiling.
  • ChIP-seq and CUT&RUN for protein-DNA interaction mapping.
• Cancer Research
  • Tumor mutational burden (TMB) analysis.
  • Detection of somatic mutations, copy number variations, and fusions.

NGS Kits are indispensable tools in modern molecular biology, providing robust solutions for a wide range of genomic and transcriptomic studies.