AffiNGS® CUT&RUN m6A RNA Enrichment (MeRIP) Kit

AffiNGS® CUT&RUN m6A RNA Enrichment (MeRIP) Kit

The kit enriches RNA fragments containing m6A for region-specific identification via PCR or epitranscriptome-wide profiling using next-gen sequencing.

Features:

• High enrichment: Targets m6A with minimal background.
• Low input: Effective with as little as 500 ng RNA.
• Fast: Procedure completes in approximately 2.5 hours.
• Convenient: All necessary components included.

Components:

Includes wash buffers, antibodies, proteinase K, RNA binding beads, and other essential reagents.

Protocol Overview:

1. Immunocapture and Cleavage: Mix RNA with antibodies and beads, incubate, add cleavage enzyme.
2. Enriched RNA Release/Recovery: Digest proteins, purify RNA, bind to beads, wash, elute RNA.

Applications:

Used in research to understand m6A RNA methylation's role in biological processes and disease.

Storage:

Kit components should be stored at specified temperatures, stable for up to 6 months.