AffiNGS® CUT&RUN m6A RNA Enrichment (MeRIP) Kit
CAT# AFG-PNG-16
Size: 24 reactions
AffiNGS® CUT&RUN m6A RNA Enrichment (MeRIP) Kit
The kit enriches RNA fragments containing m6A for region-specific identification via PCR or epitranscriptome-wide profiling using next-gen sequencing.
Features:
- High enrichment: Targets m6A with minimal background.
- Low input: Effective with as little as 500 ng RNA.
- Fast: Procedure completes in approximately 2.5 hours.
- Convenient: All necessary components included.
Components:
Includes wash buffers, antibodies, proteinase K, RNA binding beads, and other essential reagents.
Protocol Overview:
- Immunocapture and Cleavage: Mix RNA with antibodies and beads, incubate, add cleavage enzyme.
- Enriched RNA Release/Recovery: Digest proteins, purify RNA, bind to beads, wash, elute RNA.
Applications:
Used in research to understand m6A RNA methylation's role in biological processes and disease.
Storage:
Kit components should be stored at specified temperatures, stable for up to 6 months.