CUT & RUN Assay Kits

Cleavage Under Targets and Release Using Nuclease (CUT&RUN) is an advanced epigenomics technique designed to map protein-DNA interactions with high precision and minimal input requirements. This method utilizes antibody-directed targeting of a protein of interest, followed by enzymatic cleavage of DNA near binding sites. CUT&RUN is increasingly popular due to its sensitivity, simplicity, and reduced sequencing cost compared to traditional methods like ChIP-seq.

Content

• Principle of CUT&RUN
  CUT&RUN combines the specificity of antibodies with the efficiency of enzymatic cleavage to profile protein-DNA interactions. The workflow involves:
  • Immobilization of cells or nuclei on a solid support.
  • Antibody binding to the target protein of interest.
  • Tethering of a Protein A/MNase fusion enzyme to the antibody.
  • Controlled activation of MNase to cleave DNA proximal to the binding sites.
  • Release of the cleaved DNA fragments for downstream sequencing.
• Key Features
  • High sensitivity: Detects protein-DNA interactions from as few as 100–1,000 cells.
  • Reduced sequencing depth: Enriched signal minimizes background noise.
  • Compatibility: Works with diverse samples, including primary cells and tissues.
  • Simplified workflow: Reduced experimental time and complexity.

Applications of CUT&RUN Assay Kits

CUT&RUN is widely used in molecular biology, epigenetics, and cancer research. Some of its specific applications include:

• Histone Modification Mapping: Identifying specific modifications like H3K27me3 or H3K4me3 to study chromatin states.
• Transcription Factor Studies: Determining binding sites of transcription factors such as CTCF, Oct4, or Sox2.
• Disease Research: Exploring epigenetic changes in diseases such as cancer, neurodegenerative disorders, and autoimmune conditions.
• Drug Discovery: Evaluating the impact of epigenetic drugs on protein-DNA interactions.
• Developmental Biology: Studying dynamic chromatin changes during development or differentiation.

CUT&RUN Assay Kits provide a robust and cost-effective solution for precise epigenomic studies, enabling researchers to explore complex gene regulatory mechanisms with high accuracy and efficiency.