CUT&RUN Assay Kits AffiNGS

CUT&RUN (Cleavage Under Targets and Release Using Nuclease) is a technique designed for high-resolution mapping of protein-DNA interactions. The AffiNGS CUT&RUN assay kits are specifically tailored for enhanced precision and sensitivity in chromatin immuno-precipitation assays.

Nuclease and Antibody Specificity

• The kit includes a highly specific micrococcal nuclease (MNase) that is utilized for targeted cleavage of DNA adjacent to bound proteins.
• Customizable antibodies are included, allowing for specific targeting of protein-DNA interactions.

Buffer Components

• Various buffers are provided for cell lysis, chromatin digestion, and reaction termination, including:
  • Lysis Buffer: For cell and nucleus lysis.
  • Digestion Buffer: To facilitate MNase activity and target cleavage.
  • Stop Buffer: To halt the reaction and stabilize DNA fragments.

Control Elements

• Positive and negative controls to validate assay performance and specificity.
• Control antibodies and chromatin preparation for troubleshooting.

Reagents for Analysis

• DNA isolation and purification reagents.
• Quantitative PCR (qPCR) or sequencing preparation reagents for downstream analysis.

Protocol Guidelines

• Detailed procedural instructions for sample preparation, digestion, and data analysis.
• Recommendations for optimizing antibody and enzyme concentrations.

The AffiNGS CUT&RUN Assay Kits are engineered for efficient and accurate mapping of protein-DNA interactions, leveraging advanced nuclease technology and tailored reagents for high-resolution epigenetic studies.